Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
Component |
Type Strain |
Volume |
Biosafety Level |
Composition |
Product Format |
Storage |
Storage Conditions |
Lysis Buffer |
梅經(jīng)理 |
17280875617 |
1438578920 |
胡經(jīng)理 |
13345964880 |
2438244627 |
周經(jīng)理 |
17757487661 |
1296385441 |
于經(jīng)理 |
18067160830 |
2088210172 |
沈經(jīng)理 |
19548299266 |
2662369050 |
李經(jīng)理 |
13626845108 |
972239479 |
改则县|
云安县|
榆社县|
建始县|
南木林县|
万荣县|
明星|
渝中区|
玛曲县|
温州市|
三明市|
获嘉县|
久治县|
新建县|
南城县|
曲松县|
新营市|
合水县|
洛扎县|
灵川县|
长顺县|
南宁市|
罗江县|
昭通市|
故城县|
扶沟县|
庆安县|
巩留县|
黄大仙区|
中西区|
嘉鱼县|
盖州市|
肥乡县|
安达市|
东海县|
兴文县|
墨江|
红桥区|
阿勒泰市|
观塘区|
晋宁县|
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