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2.43
2.43
規(guī)格:
貨期:
編號:B163630
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

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凍干粉
斜面
甘油
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產(chǎn)品名稱 2.43
商品貨號 B163630
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue Spleen
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain Lewis (B cell); BALB/c (myeloma)
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with CTL Clone L3 cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse cytotoxic, suppressor T cell antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse cytotoxic, suppressor T cell antigen)
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 104 cells/mL and maintain between 4 x 104 and 5 x 105 cells/mL. 

Medium Renewal: Every 2 to 3 days

Cryopreservation Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Isotype IgG2b
Name of Depositor FW Fitch
Deposited As rat (B cell); mouse (myeloma)
References

Sarmiento M, et al. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing lyt 2 antigen block T-cell mediated cytolysis in the absence of complement. J. Immunol. 125: 2665-2672, 1980. PubMed: 6159417

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Sarmiento M, et al. Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis. Immunol. Rev. 68: 135-169, 1982. PubMed: 6184304

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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