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7-34-1
7-34-1
規(guī)格:
貨期:
編號(hào):B163812
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) 7-34-1
商品貨號(hào) B163812
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic)
Cellular Products
immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic)
Comments
Animals were immunized with peripheral blood mononuclear cells (PMNC) from SLA c haplotype miniature swine.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific.
It does not cross-react with PMNC from humans, sheep, goats or mice, but does cross-react with cells from commercial varieties of pigs.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor CM Warner
Deposited As mouse (B cell); mouse (myeloma)
References

Lie WR, et al. Preparation and characterization of murine monoclonal antibodies to swine lymphocyte antigens. Immunology 64: 599-605, 1988. PubMed: 2459049

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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
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