国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > DEC-205 [NLDC-145]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
DEC-205 [NLDC-145]
DEC-205 [NLDC-145]
規(guī)格:
貨期:
編號:B164343
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DEC-205 [NLDC-145]
商品貨號 B164343
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with mouse lymph node stroma.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen)
Comments

The antibody reacts with an antigen that is expressed at high levels on mouse dendritic cells and thymic cortical epithelial cells.

The antigen is a 205,000 dalton group VI C type molecule that mediates adsorptive endocytosis and antigen presentation.

The antibody reacts with and stains mouse dendritic cells and epidermal Langerhans cells.

The cells are capable of being adapted to serum free growth in using Nutridoma medium.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 2 x 10and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2a
Name of Depositor RM Steinman, G Kraal
Deposited As rat (B cell); mouse (myeloma)
References

Kraal G, et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163: 981-997, 1986. PubMed: 3950549

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
安阳市| 江阴市| 广东省| 华容县| 阿合奇县| 康乐县| 乌拉特前旗| 黄陵县| 巍山| 龙口市| 浦北县| 柯坪县| 高安市| 阜康市| 额济纳旗| 民权县| 九江县| 津南区| 哈密市| 胶州市| 武定县| 阜康市| 安宁市| 永登县| 汉川市| 句容市| 遵义县| 阳泉市| 泾阳县| 松江区| 江北区| 太原市| 会同县| 阿克| 同心县| 龙泉市| 昆山市| 赤城县| 连平县| 延寿县| 五台县|