国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pHK9
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pHK9
pHK9
規(guī)格:
貨期:
編號:B231748
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pHK9
商品貨號 B231748
Designations pHK9
Depositors F Spencer
Biosafety Level 1
Vector Information
Size (kb): 10.8999996185302700
Vector: pHK9 (plasmid)
Marker(s):ampR
Construct size (kb): 10.89999961853027
Features: marker(s): ampR, LYS2
replicon: ori
MCS: SalI (delta15)
restriction site: EcoRI,BamHI,XhoI,HpaI,NruI,BglII,PstI
restriction site: PvuII
coding sequence: karldelta15
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.9; EcoRI--6.5, 2.4, 2.05; PstI--9.0, 1.8; PvuII--10.9.
Restriction digests of the clone give the following sizes (kb): BglII--7.8; EcoRI--7.8; HindIII--5.2, 2.5; EcoRI/HindIII--5.2, 2.5.
Supports introduction of kar1 delta 15 mutation into lys2 yeast strains by two step gene replacement. The karl delta 15 mutation supports transfer of YAC clones between yeast strains by Karl-mating (REF).
To integrate pHK9, linearize within the kar1 gene at the unique PvuII site. Cells that have lost the LYS2 marker through mitotic excision can be selected on alpha- aminoadipate.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vallen EA, et al. Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion. J. Cell Biol. 117: 1277-1287, 1992. PubMed: 1607389

Spencer F, et al. Yeast kar1 mutants provide an effective method for YAC transfer to new hosts. Genomics 22: 118-126, 1994. PubMed: 7959757

Hugerat Y, et al. A versatile method for efficient YAC transfer between any two strains. Genomics 22: 108-117, 1994. PubMed: 7959756

Spencer F, Simchen GTransfer of YAC clones to new hosts by karyogamy deficient mating.In: Spencer F, Simchen GCurrent Protocols in Human GeneticsNew York, N.Y.John Wiley and Sonsin press, 1995

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
柳州市| 罗城| 双峰县| 东乌珠穆沁旗| 梅河口市| 吕梁市| 平塘县| 比如县| 塔河县| 浦江县| 香港| 塔城市| 遵义市| 保康县| 五大连池市| 湘潭县| 仙桃市| 宁蒗| 喜德县| 武冈市| 洞头县| 邵阳县| 大渡口区| 香河县| 海城市| 阳曲县| 景德镇市| 左权县| 牟定县| 老河口市| 礼泉县| 峨眉山市| 丹江口市| 遂川县| 自贡市| 陵川县| 铁力市| 宾川县| 东宁县| 临夏市| 台山市|