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P24
P24
規(guī)格:
貨期:
編號:B237474
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 P24
商品貨號 B237474
Designations P24
GenBank Number

J04211

Species Homo sapiens, human
Applications
This probe encodes an epitope of collagen type VI since it was identified by immunoscreening a lambda gt11 library.
Vector
Construct size (kb): 4.5
Insert
DNA: cDNA
Insert lengths(kb): 1.5
Tissue: placenta
Gene product: collagen, type VI, alpha 3 [COL6A3]
Alleles: A1, A2, B1, B2
Insert Size (kb) 1.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BamHI--4.5; PstI--3.0, 1.5; EcoRI/HindIII--3.0, 1.5; SalI--4.5.
The insert begins at about nucleotide 6000 of the published sequence and includes an XhoI site at 6043 and a PstI site at 7339.
This probe encodes an epitope of collagen type VI since it was identified by immunoscreening a lambda gt11 library.
The insert encodes approximately the 3' 900 bp of the triple-helical domain and sequences 3' to that.
This probe detects the following genomic TaqI fragments (kb): Human--5.6, 3.0, 2.5, 2.3, 2.0, 1.8; Chinese hamster--6.6, 3.0; mouse--4.4, 3.0.
This probe detects mRNAs of about 8.5 to 9.2 kb in human fibroblasts.
References

Brotherton D, et al. An NcoI RFLP associated with the gene encoding the alpha-3 chain of human type VI collagen (COL6A3). Nucleic Acids Res. 17: 1274, 1989. PubMed: 2564183

Chu ML, et al. Amino acid sequence of the triple-helical domain of human collagen type VI. J. Biol. Chem. 263: 18601-18606, 1988. PubMed: 3198591

Weaver EJ, et al. A BglI polymorphism in the type VI (alpha3) procollagen gene [COL6A3]. Nucleic Acids Res. 16: 11386, 1988. PubMed: 2905051

Chu ML, et al. Mosaic structure of globular domains in the human type VI collagen alpha3 chain: similarity to von Willebrand factor, fibronectin, actin, salivary proteins and aprotinin type protease inhibitors. EMBO J. 9: 385-393, 1990. PubMed: 1689238

Weil D, et al. Cloning and chromosomal localization of human genes encoding the three chains of type VI collagen. Am. J. Hum. Genet. 42: 435-445, 1988. PubMed: 3348212

Mon-Li Chu, personal communication

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